Which step is the first in translation termination?

Which step is the first in translation termination?

• A. Binding of release factor to stop codon
• B. Release of polypeptide
• C. Release of tRNA
• D. Disassembly of ribosome

Answer: (A)

Termination begins when a stop codon in the ribosome’s A site is recognized by a release factor. This recognition acts as the trigger to start the termination process because release factors are the factors that specifically bind the stop codon and catalyze the release of the finished polypeptide. Once bound, the release factor promotes hydrolysis of the bond between the polypeptide and the tRNA in the P site, freeing the newly made protein. After that, the tRNA is released from the P site, and the ribosome dissociates into its subunits with the help of other factors. So, recognizing the stop codon by a release factor is the first essential step; the polypeptide release, tRNA release, and ribosome disassembly follow in sequence.

Termination begins when a stop codon in the ribosome’s A site is recognized by a release factor. This recognition acts as the trigger to start the termination process because release factors are the factors that specifically bind the stop codon and catalyze the release of the finished polypeptide. Once bound, the release factor promotes hydrolysis of the bond between the polypeptide and the tRNA in the P site, freeing the newly made protein. After that, the tRNA is released from the P site, and the ribosome dissociates into its subunits with the help of other factors. So, recognizing the stop codon by a release factor is the first essential step; the polypeptide release, tRNA release, and ribosome disassembly follow in sequence.